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KMID : 0364820220580030157
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Korean Journal of Microbiology
2022 Volume.58 No. 3 p.157 ~ p.168
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Optimization of culture condition for diazinon-degrading bacterium Paenibacillus tritici strain PH55-1 using response surface methodology
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Kim Ye-Eun
Song Jae-Kyeong
Ahn Jae-Hyung
An Si-Hyun
Weon Hang-Yeon
Kim Jeong-Jun
Chung Joon-Hui
Yeon Je-Hyung
Kim Da-Yeon
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Abstract
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The main objective of this study was to optimize the culture condition for enhanced growth of Paenibacillus tritici strain PH55-1 which was reported as the diazinon biodegradable microorganism having several genes related to degradation of organophosphorus pesticides in the previous study. In this study, the optimized media composition and culture conditions were explored by using the response surface methodology.
Through the Plackett-Burman design (PBD), D-mannitol, defatted soy flour, and molybdenum were verified as the best medium components, and the temperature was confirmed as most important environmental factor of the growth of strain PH55-1. The central composite design (CCD) predicted the optimal concentration of D-mannitol, defatted soy flour, and molybdenum were 3.7%, 5.46%, and 2.2 mM, respectively, and the optimal temperature was 33.8°C for optimal growth of strain PH55-1. The predicted maximum bacterial growth of strain PH55-1 was 9.67 × 109 CFU/ml in the optimum condition, and the confirmed cell density by the subsequent validation experiment was 3.21 × 109 CFU/ml, which was 13.3 times higher than that of the commercial medium, Tryptic Soy Broth (TSB; 2.42 × 108 CFU/ml) as the control medium. Improving the culture condition for P. tritici strain PH55-1 could benefit for the industrial application of bio-remediation technology to organophosphate pesticide- contaminated agricultural fields.
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KEYWORD
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biodegradation, culture condition, diazinon, optimization, response surface methodology
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